University of Khartoum

Types and Load of Bacteria Contaminating Drinking Water in Dairy Farms in Khartoum North

Types and Load of Bacteria Contaminating Drinking Water in Dairy Farms in Khartoum North

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Title: Types and Load of Bacteria Contaminating Drinking Water in Dairy Farms in Khartoum North
Author: Babeker, Shirin
Abstract: This study was conducted to assess the bacterial contamination of drinking water in dairy farms in Khartoum North, based on bacterial isolation and identification and viable count. A total of 33 farms were randomly selected. Three samples were taken from each farm, one from the main source of water (network or well), the second from the surface of water in drinking trough and the third from the wall of water trough. In addition, five samples were taken from storage places of water (found in only five farms), making the total number of samples 104. All samples were cultured on Blood Agar and MacConkey’s Agar for bacterial isolation and on Nutrient Agar for viable counts. A total of 188 isolateswere obtained, 57% of them were Gram-positive bacteria. The isolates were identified according to their microscopic, cultural and biochemical properties to 19 bacterial species. The species and their isolation percentages related to number of samples were Corynebacterium renale(29.8%), Aeromonas salmonicida(17.3%), Micrococcus luteus (16.3%), Klebsiella pneumoniae spp.aerogenes (14.4%), Aeromonas spp. (13.5%), Bacillus mycoides(12.5%) Escherichia coli (11.5%), Corynebacterium pseudodiphtheriticum(10.6%), Staphylococcus epidermidis(8.7%), Moraxella urethralis (8.7%), Nocardia asteroides(6.7%), Corynebacterium diphtheriae(5.8%), Proteus spp. (5.8%), Bacillus sphaericus (5.8%), Staphylococcus warneri (4.8%), Aeromonas sobria(2.9%),Kingella kingae(2.9%), Micrococcus lylae ( 1.9%) and Klebsiella oxytoca (1.0%). Water samples from troughs were the most contaminated by all bacterial species except Klebsiella oxytoca, followed by the samples from wallsof water troughs and the least contaminated was the main source of water. Aeromonas salmonicida, XIV Bacillus mycoides, Bacillus sphaericus andMicrococcus luteus were found in all water sources, whereas Micrococcus lylaeand Kingella kingae were found only in water of troughs and Klebsiella oxytoca (one isolate) was found in a wall of a trough. The results of the viable count showed considerable variation among sources ofwater. The mean viable count was 4x10 3 , 4.4x10 5 , 1x10 6 and5.2x10 6 cfu/ml for the main source, storage places, surfaces of water in troughs and wall of troughs, respectively. In conclusion, many bacterial species and high viable counts were found in drinking water of dairy cattle in Khartoum North, some of them are waterborne pathogens this finding points to poor hygienic measures which constitute hazards to dairy production and public health.
URI: http://khartoumspace.uofk.edu/handle/123456789/9101
Date: 2015-04-18


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