University of Khartoum

Antidotal Effect of Datura stramonium on the Toxicity of Carbofuran and Malathion Insecticides in Rats

Antidotal Effect of Datura stramonium on the Toxicity of Carbofuran and Malathion Insecticides in Rats

Show full item record

Title: Antidotal Effect of Datura stramonium on the Toxicity of Carbofuran and Malathion Insecticides in Rats
Author: Yahia, Zuhour
Abstract: The purpose of the present study was to determine the antidotal effect of Datura stramonium(aqueous seed extract) (DSE) in the treatment of toxicity of two types of pesticides: carbofuran and malathion. In the first experiment, (DSE) was used as a pretreatment tocarbofuran toxicity compared to a reference drug (Atropine sulphate).It was studied into twentyfive albino rats arranged into five groups. The first Group dosed carbofuran 10 mg/kg. The second, third and fourth groups received 5, 7.5mg /kg of (DSE) and Atropine sulphate of 17mg/kg respectively. After the administration of (DSE) and the drug, in the second, third and fourth groups they received carbofuran 10 mg/kg body weight. The fifth group was a control. The second experiment was to determine the antidotal effect of (DSE) in treatment of malathion toxicity. It was studied into twenty-five albino rats arranged into five groups. Four groups (group one, two, three and four) received 500mg/kg body weight of Malthion. After the administration of malathion in the four groups they received 5, 7.5mg /kg of (DSE) and 17mg/kg of Atropine sulphate respectively. The fifth group was a control. In the two experiments, blood sample was collected for assay of serum enzymes and metabolic indicators. The rats weresacrificed after twenty-four hours. Tissue specimens were processed for histopathology. The result of histopathology and biochemical in both experiments showed normal internal organs and normal levels of enzymes in the groups dosed 7.5 mg/kg of (DSE) and the drug. The groups received 5mg /kg of (DSE) showed mild toxicity in the internal organ and slightly high levels of serum enzymes. The groups dosed the two insecticides only showed typical toxicity in the internal organs and high level of serum enzymes. This reveals that the (DSE) XII of 7.5 mg/kg has almost the same effect ofthe drug in treatment of both toxicities and indicates that the protective action of (DSE) against the two insecticides tested was dose dependant. The third experiment was designed to estimate the safety of higher doses of (DSE). It was studied into sixteen whiterats arranged into four groups. Three groups received 7.5, 15 and 30 mg/kg of (DSE) respectively. The fourth was a control. The rats were sacrificed after twenty four hours. Blood sample was collected for analysis of serum enzymes and metabolic indicators. Tissues were processed for histopathology. The ratsdosed 15and 30 mg/kg of (DSE) showed typical toxicity in the internal organs with elevated level of serum enzymes. Rats received 7.5 mg/kg of (DSE) showednormal internal organs and normal levels of serum enzymes. These suggest that increasing the dose of (DSE) above the 7.5mg/ kg could be toxic to animals and indicate the safety of (DSE) 7.5 mg /kg dose in treatment of insecticides toxicity.
URI: http://khartoumspace.uofk.edu/handle/123456789/9141
Date: 2015-04-21


Files in this item

Files Size Format View

This item appears in the following Collection(s)

Show full item record

Share

Search DSpace


Browse

My Account