University of Khartoum

Identification of Aerobic Bacteria Isolated From Vagina of Cross-Bred Dairy Cows during Early Postpartum In University Farm, Khartoum North, Sudan

Identification of Aerobic Bacteria Isolated From Vagina of Cross-Bred Dairy Cows during Early Postpartum In University Farm, Khartoum North, Sudan

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Title: Identification of Aerobic Bacteria Isolated From Vagina of Cross-Bred Dairy Cows during Early Postpartum In University Farm, Khartoum North, Sudan
Author: Bashir Mohmoud Sadig, Nahid
Abstract: A study was carried out to identify thebacteria which are found in the reproductive tract of dairy cows during parturient and to find the appropriate treatment. The area of study was Khartoum University Farm at Shambat, Khartoum North, Khartoum State. The cows examinedwere 4 – 8 years old cross-bred dairy cows (Friesian × Kenana). A total of 80 vaginal swabs were collected from 55 postparturient cows. These swabs were collected within 3 days after delivery. Thirty vaginal swabs were collected 1 – 3 days after parturition. Twenty – five vaginal swabs were collected from cows 1-3 days after parturition before treatment with antiseptic 1% Lugol's iodine and then vaginal swabs were collected from the same twenty – five cows 24 hours after treatment with the antiseptic 1%Lugol's iodine. The vagina of postparturient cow was sampled by sterile cotton wool. The collected swabs were inoculated on blood agar and MacConkey's agar. The isolated bacteria were identified by their cellular morphology, arrangement, staining reaction and biochemicalproperties. The antibacterial sensitivity of the isolated bacteria was examined to Ampicillin, Gentamysin, Chloramphincol, Cloxacillin, Erythromycin, Pencillin, Tetracycline, Streptomycin, Naladixic acid, Nitrofurantol, Cloistin and Cotrimoxazole and 1% Lugol'siodine. The indentified bacteria were Staphylococcus, Streptococcus, Corynobacterium, Bacillus, Escherichia, Kelbsielle, Aerococcus, Micrococcus and Proteus. The Gram positive bacteria isolated from 30 postpartum vaginal swabs within 1-3 days after parturition were Staphylococcus spp, Streptococcus spp,Bacillus spp, Micrococcus spp, Aerococcus spp, Lactobacillusspp and Corynobacterium spp. The Gram- positive bacteria isolated from 25 postpartum vaginal swabs before treatment with 1% Lugol’s Iodine wereStaphylococcus spp, Streptococcus spp, Bacillus spp, Micrococcus spp and Corynobacteriumspp. The Gram-positive bacteria isolated from 25 postpartum vaginalswabs after 1-3 days after treatment with 1% Lugol’s Iodine were Staphylococcus spp, Streptococcus spp, Bacillus spp, and Corynobacteriumspp. The Gram- negative bacteria isolated from 30 postpartum vaginal swabs within 1 – 3 days after parturition were E. coli, Klebsiella spp, Protues spp, Enterobacter spp. The Gram- negative bacteria isolated from 25 postpartum vaginal swabs before treatmentwith 1% Lugol’s Iodine were E.coli, Klebsiella spp and Protues spp. The Gram- negative bacteria iv isolated from 25 postpartum vaginal swabs after treatment with 1% Lugol’s Iodine were E.coli, Klebsiella spp and Protues spp.The sensitivity ofthe isolates to antibacterial agents was variable. They were less sensitive to the commonly used drugs like Tetracycline and Cloxacillin. This may be due to misuse of these drugs, while they were highly sensitive to others like Gentamysin and Ampicillin which are less commonly used. The sensitivity of the isolates to 1%Lugol's iodine was variable. Some isolates were resistant and a few were sensitive.
URI: http://khartoumspace.uofk.edu/handle/123456789/9381
Date: 2015-04-23


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