University of Khartoum

Serological Survey of Cattle Brucellosis in Eldein, East Darfur State, Sudan

Serological Survey of Cattle Brucellosis in Eldein, East Darfur State, Sudan

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Title: Serological Survey of Cattle Brucellosis in Eldein, East Darfur State, Sudan
Author: Abdalla Senein Musa, Mahmoud
Abstract: The study was carried out to determine the prevalence of the disease in cattle in Eldein area, East Darfur State, Western Sudan. Two hundred and fifty serum samples were collected from 8 herds and different local breeds of Bagara cattle (Rizigat, Ambrarrow) , Kenana, Butana, and Cross Fresian with different sex and ages from differen locations (Eldein, Abujabra, Bahar Elarb, Asalaia and Elfardoas). The results showed 21 (8.4%) positive using Rose Bengal Plate Test (RBPT). Serum Agglutination Test (SAT) showed 50 (20%) positive and Competitive Enzyme Linked Immuno Sorbent Assay (cELISA) showed 5 (2%) positive. The history of cases indicated that 24 (9.6%) aborted cows, 20 (8%) with histories of retained placentas and 3 (1.2%) cases of knee hygromas. The results revealed that sex, age and breed were not associated with the disease according to statistical analysis (P>0.05). There was a significants difference (P<0.05) between serological tests. Using agreement between tests (Kappa Statistic) indicated that RBPT and SAT had moderate agreement (Kappa=0.547), RBPT and cELISA had fair agreement (Kappa=0.364) and SATand cELISA had slight agreement (Kappa=0.158). cELISA is the most sensitive and reliable test in field
URI: http://khartoumspace.uofk.edu/handle/123456789/9702
Date: 2015-04-27


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