Phytochemical, Biological and Formulation Studies of Three Local Medicinal Plants
Phytochemical, Biological and Formulation Studies of Three Local Medicinal Plants
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Date
2015-04-03
Authors
Himmed, Mohammed
Journal Title
Journal ISSN
Volume Title
Publisher
UOFK
Abstract
The present study was carried out on three types of Sudanese medicinal
plants (Ambrosia maritima, Corn-silk and Cymbopogon proximus) used
for dissolution of urinary calculi (kidney, ureter and bladder), and also
diuretic.
Ambrosia maritima:
Phytochemical screening, toxicity and biological activities were carried
out. Ambrosia maritima showed toxic effect but not lethal.
Pharmacological studies of Ambrosia maritima showed
relaxation/contraction on isolated rabbit intestine. Local anaesthetic
effects of Ambrosia maritima were studied using frog foot-withdrawal
reflex method, and compared with benzocain (refrence drug). The results
showned that, Ambrosia maritima has no local anaesthetic effect.
Ambrosia maritima was tested against gram negative bacteria,
Pseudomonas, E. coli, Klebsiella Spp. and gram positive bacteria
Staphylococcus aureus, the results obtained showed that Ambrosia
maritima has no antibacterial activity against either gram negative or
gram positive type.
Ambrosia maritimawater extract was formulatedin tablet dosage form
and the physicochemical properties; hardness (4.6 kg /in
2
) , friability
(0.6%), disintegration (12 min) and dissolution (u.v reading after 45 min:
0.204) were determined and evaluated , these results comply with U.S.P
and B.P requirements.
Corn-silk:
Phytochemical screening of Corn-silk showed presence of alkaloids,
flavonoids, saponins and tannins. Saponins and flavonoidswere separated
using chromatographic technique, butaqueous extract was used as it is
similar to its oral use in folk medicine.
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Pharmacological experiments were conducted using experimental animal
(Rabbit, Local). Corn-silk water extract showed contraction/relaxation
effects at different concentrations.
Local anaesthetic effects of Corn-silkwere studied. The results obtained
showed that, Corn- silkhas no local anaesthetic effect.
Antibacterial activity was tested and the results obtained showed that, the
plant under test has no effect on Escherichia coli, klebsiella spp.,
Pseudomonas spp. and Staphylococcus aureus.
Aqueous extract of Corn-silkwas prepared and formulated in tablet
dosage form and subjected to a number of quality control tests, including
physicochemical parameters; hardness (4.2 kg / in
2
), friability (0.7 %),
disintegration (13 min) and dissolution (u.v reading after 45 min: 0.680).
The results obtained where within the range stated in B.P and U.S.P.
pharmacopoeia .
Cymbopogon Proximus
Pipretone was separated using chromatographic technique, particularly,
thin layer chromatography and columnchromatography. The struture was
illucidated by infra-red spectroscopy.
Antibacterial activity of Cymbopogon proximus gave negative results
against both gram negative bacteria (Pseudomonas, Klebsiella, E. coli),
and gram positive bacteria (Staphylococcus spp.).
Combined tablets were formulated from Ambrosia maritima, Corn-silk
andCymbopogon proximus. Physicochemical properties; hardness (4.6
kg/in
2
) , disintegration (12 min), friability (081 %) and dissolution (u.v.
reading after 45 min : 0.220) were recorded and evaluated .These results
comply with U.S.P and B.P pharmacopoeia .
Clinical Studies:
Clinical Studies were conducted to prove the performance and to evaluate
the therapeutic activities of Corn- silk. In this study, sixty patients with
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different complaints mainly urinary track infection received herbal
treatment (decoction), more than sixty percent of the patients were cured
by this herb. For the above activity, Corn-silkwas formulated in tablets
dosage form.
Description
215page
Keywords
Phytochemical, Biological,Formulation Studies,Local Medicinal Plants