Isolation And Characterization Of Microorganisms Capable Of Degrading Crude Petroleum Oil Under Aerobic Conditions

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Date
2015-06-15
Authors
Elkhir, Nada Mohammed
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Publisher
UOFK
Abstract
In this study a total of one hundred and thirty five strains of petroleum degrading bacteria and a few fungi were isolated from 70 soil samples taken from different oil polluted areas in Khartoum State and 10 water samples from Hegleg. The isolates obtained from soil samples collected from an area contaminated for a long time showed higher degradation rates of petroleum than isolates from soils contaminated for a short time. The isolates were considered good oil degraders. They were able to degrade n–alkane more than total petroleum hydrocarbons. Six of these isolates showed a high degree of degradation which was studied by GC (Gas chromatography). Isolate W.14.28 (Pseudomonas mendocina) showed the highest percent of degradation (96.79%) of n-alkanes of chain length (C1-C42) followed by isolate W.18.34 (Pseudomonas putida) (95.67%), isolate C.3.4. (Pseudomonas. aeruginosa) (95.74%), isolate W.20.39 (94.61%), (Pseudomonas putida), isolate F.35.70 (Bacillus brevis) 91.97% and isolate W.15.29 (Burkholderia cepacia) (85.2%). Combinations of the best six isolates gave high percentages of degradation than single strains. Physiological studies have shown that the best 20 isolates were mesophilic .With respect to salt tolerance, only isolate C.7.12 (Micrococcus roseus) was able grow at 10%NaCl.
Description
158page
Keywords
Petroleum Oil
Citation
University of khartoum
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