ISOLATION OF COLIFORMS , ESHERICHIA COLI AND OTHER AEROBIC BACTERIA from PASTEURIZED MILK SAMPLES IN KHARTOUM AND KHARTIUM NORTH

Abstract

The present study was to investigate the presence of coliforms and E. coli and determine the bacterial contamination of pasteurized milk samples in Khartoum state Thirty packaged pasteurized milk samples were collected from Khartoum state shops . The samples were examined microscopically and cultured to determine if there were coliforms and E. coli bacteria and also to determine the types of bacteria involved in pasteurized milk samples . Twenty six samples (86.7%) of milk was found to be positive for bacteria . Were as only six (13.3%) gave negative bacterial culture. The isolated bacteria from pasteurized milk were Staphylococcus (82%), Enterobacteria (15.4%) and Pasteurella(2.6%) Further more the species of staphylococcus isolated were haemolyticus(12.8 %) , simulans(12.8%), capitis (9%), aureus(10.3%), caseolyticus(7.7%), epidermidis (7.7%), hominis (6.4) , intermidius (6.4%), chromogenes (2.6%), saprophticus(5%) and ludgunensis (1.3%) The bacteria which was isolated from milk samples other than Staphylococcus species were serratia marcescens (7.7%), E .coli (2.6%), proteus mirabilis (3.8%), enterobacter aerogenes (1.3%) pseudomonas pseudomallei (1.3%) and pasteurella haemolytica (2.6%) The counts of total coliforms were found to range between 0.9××103.and 6.4 ×103 The present study concluded that the pasteurized milk distributed in Khartoum State was of low quality . Hence it was recommended that quality assurance programmes should be started to ensure that good quality milk and milk products are produced and consumed in the country.