ROLE OF IFN-γ AND IFNGR1 GENE POLYMORPHISMS IN SUSCEPTIBILTY TO POST KALA-AZAR DERMAL LEISHMANIASIS (PKDL) IN SELECTED SUDANESE POPULATION
ROLE OF IFN-γ AND IFNGR1 GENE POLYMORPHISMS IN SUSCEPTIBILTY TO POST KALA-AZAR DERMAL LEISHMANIASIS (PKDL) IN SELECTED SUDANESE POPULATION
No Thumbnail Available
Date
2006-07
Authors
Ahmed, Mohamed
Salah Eldin, Hiba
Journal Title
Journal ISSN
Volume Title
Publisher
University of Khartoum
Abstract
The aim of this study is to determine whether IFNGR1 and IFN-γ gene
polymorphisms are associated with PKDL in the high susceptible Masalit tribe from
three adjacent villages Um-salala, EL-Rugab and Ali babkir in eastern Sudan. DNA
was prepared from 62 Trios and 32 pairs using Multiple Displacement Amplification
(MDA) method. Four IFNGR1 polymorphisms; +95T/C, -56T/C, -270T/C and -470
ins/del and two IFN-γ polymorphisms; +874T/A and -179G/A were genotyped using
ARMS-PCR and RFLP. The data were tested for their fit to Hardy- Weinberg
equilibrium, and the polymorphisms were analyzed for their association to the disease
using TDT, single point logistic regression analysis and Haplotype base score test. All
polymorphisms are found in Hardy- Weinberg equilibrium. TDT analysis showed an
evidence of association between PKDL and IFNGR1-270 polymorphism (P=0.023;
X2=4.571). The haplotype base score test indicates that -270 polymorphism has
separate main effect on PKDL (P=0.008). No evidence of association between other
SNPs and susceptibility to PKDL. The frequency of IFN-γ polymorphism +874 allele
was low among Masalit tribe (0.2).
In conclusion -270T/C polymorphism may have functional affect or in linkage
disequilibrium with other functional SNPs within IFNGR1 gene
Description
Keywords
leishmaniasis,Immunology,Transmission disequilibrium testing(TDT),DNA Quantification