Cytogenetic And Molecular Identification Of The Anopheles Gambiae Complex (Giles) Members From Western Sudan States

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El hag, Ihsan Abdelrazig Elfak
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Malaria is recognised as one of the most severe health hazards in Sudan. The serious socio- economic impact of malaria on public health is a major hindrance to development programmes in most parts of the country. Mosquitoes of the Anopheles gambiae Giles, namely Anopheles arabiensis and Anopheles gambiae are the two major vectors of malaria in Sudan. The two sibling species are widespread throughout Sudan and hence sympatric in most areas of the country. This study includes both field and laboratory components. Anopheline mosquitoes were collected from specific sites in Western Sudan using standard methods. Cytogenetic maps were constructed from salivary gland and female ovarian nurse cell polytene chromosomes. Standard giemsa and aceto-orcein staining was used to identify chromosomes. DNA extraction and PCR were used for molecular identification. Result show that all samples which were collected, at different stages of the life cycle were identified as Anopheles arabiensis. This result shows that Anopheles arabiensis has a continuous range in Western Sudan States. The cytogenetic constructions show the Anopheles arabiensis from Western Sudan is a close relative of Nigeria Anopheles arabiensis
103 Pages
Cytogenetic, Molecular Identification,Anopheles Gambiae Complex , Members,Sudan