In vitro clonal Propagation of grapevine (Vitis spp.)

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Osman, Magdoleen Gamar Eldeen
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Grapes are considered by many scientists as of the promising crops in Sudan . Its cultivation was successful under Sudan climatic conditions . The main single factor that limits grape cultivation in Sudan is the availability of stock mother plant of known varieties . This has led to the scarcity of planting material that is disease free . Introduction of plant from aboard is expensive . The objective of this study was to tailor a nutrient medium suitable for the clonal propagation of grape using tissue culture techniques . Shoot – tips and nodal cutting were cultured on various concetrations of MS salt mit (0.25X, 0.5X, 1.0X, 2.0X). The addition , potassium hypophosphate (KH2PO4) to the medium was also tried as a supplement to test its effact on growth and propagation of grapes . Various combinations of the auxin (NAA) and the cytokinin (kinetin) were tested for the propagation purpose of grapes . The auxin (IBA) was also used for the rooting of in vitro produced plantlets before transfer to soil . Nodel cutting s proved to be the ex – plant of chice for the clonal propagation of grapes when cultured on full MS salt mix . Further enhancements were gained with suppliment of KH2PO4 giving best results in all parameters measured this was adopted as the ex- plant for culture initiation in all experiments conducted in this study . The result also showed that the best combination of auxin and cytokinin for high propagation rate was 0.1mg/1 NAA and 1.0mg/1 kinitin .Best rooting on the other hand was obtianed by the addition of 0.3mg/1 IBA. Growth and development of cultured nodal cutting in liquid medium supported by cotton proved to be the best for grape propagation compared toagar or other solidifying agent .
October 2oo4
grapevine (Vitis spp.)