Assessment of Intraspecific Variation among the Snails Bulimus truncatus and Biomphalaria pfeifferi using the Random Amplified Polymorphic DNA and the Restriction Fragment Length Polymorphism Techniques

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Date
2015-07-01
Authors
Fadwa Aba Yazeed El Sayed, Osman
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Publisher
University of Khartoum
Abstract
The present study was carried out to investigate the genetic variation within the intermediate host snails of schistosomiasis in Sudan (Bulinus truncatus and Biomphalaria pfeifferi) utilizing new molecular approaches. Snails were collected from the Gezira –Managil, Seilate, Gamooeya and Faki Hashim Agricultural Schemes using the scoping method. The snails were identified morphologically using the systematic key of the Danish Bilharziasis laboratory. The random amplified DNA technique (RAPD) was used to determine the intraspecific variation in Bul. truncatus (n = 83) and Bio. pfeifferi (n = 92) using for random primer's; OPA02, OPA10, OPA11 and OPY12. The RAPD analysis showed high similarity coefficient (i.e. low genetic variation) for both Bul. truncatus and Bio. Pfeifferi. Intra-specific variation in Bul. truncatus and Bio. Pfeifferi was also assayed by polymerase chain reaction(PCR) amplification of the ribosomal internal transcribed spacer (ITS) region followed by restriction fragment length polymorphism (RFLP) analysis of this product with three restriction enzymes( RsaI , AluI, MspI). RFLP analysis of (ITS) with RsaI, AluI and MspI showed intraspecific variation in Bio. Pfeifferi. Each restriction enzyme yielded two profiles type in Bio. Pfefferi. No intraspecific variation was detected in Bul. truncatus with the three restriction enzymes. The result of the present study suggests that the RAPD and the RFLP techniques are simple, rapid and easy methods to study intraspecific variation in the intermediate host snails of schistosomiasis.
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Assessment of Intraspecific Variation among the Snails Bulimus truncatus and Biomphalaria pfeifferi using the Random Amplified Polymorphic DNA and the Restriction Fragment Length Polymorphism Techniques
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