Effect of Bacterial Metabolites Supplemented wi Gum Arabic on Cell Culture and Effect of Gum Arabic as Feed Additive in Small Ruminants

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Date
2015-04-12
Authors
Ahmed, Somaya
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Publisher
UOFK
Abstract
This study was conducted to see the effect of Gum Arabic (G.A.) on the growth of Escherichia coli, Staphylococci spp. and Klebsiella spp. when added to blood agar and RCA media. G.A. was added in concentrations of 0.5% and 1% respectively to the tested media. These media were compared with control ones without the addition of G.A. Better growth was noticed in the media to which G.A. was added and it is increased with increasing G.A. percentage. On the other hand G.A. was added to a liquid medium (RCM) in concentrations of 0.1%, 0.5% and 1% respectively. Escherichia.coli and Klebsiella spp. were cultured on the prepared media with G.A. and without G.A. as control. In a laboratory experiment, metabolites of both bacteria extracted from the media and added to kidney tissue cultures. Such cultures were compared with kidney tissue cultures to which only G.A. was added with the concentrations of 0.25% and 0.5%, and tissue culture cells without any addition of the previously mentioned materials. The results demonstrated the best growth for cells to which metabolites were supplemented with G.A. In two separate experiments G.A. dust was used as a feed additive for sheep and goats. In the first experiment 28 sheep, 6 months of age were divided into four groups of the same age and weight, (seven VIII animals in each group).For the experimental groups G.A. was added to the rations as follows: group 1 received 10% G.A., group 2 received 20% G.A. and group 3 received 30%, and accordingly the control group received no G.A. All rations were fed in a mesh form. In the second experiment 36 sheep were divided into four groups of the same age a nd weight, (9 animals in each group).The experimental design was performed as: group I received 5% G.A., group 2 received 10% G.A., group 3 received 20% G.A. and group 4 received zero G.A., as control group. Also 16 goats were divided into 2 groups, the first group received a ration to which G.A. was supplemented at a rate of 10% and the second group was left as the control group. The ration in the second experiment was prepared as pelleted ration. In both experiments parameters like body weight, blood urea , creatinine, haemoglobin, packed cell volume (PCV), total bacterial counts, and the isolation and identification of the isolated bacteria were determined. After two months from the initiation of the experiment, 3 sheep and 4 goats from each experimental group were slaughtered for the determination of warm carcass weight, chilled carcass weight and the net gross weight. IX In spite of that the results of the statistical analysis showed that differences of body weight gains were not significant, but noticeable differences between average weight gains between groups can be noticed mainly for the group with 10% G.A. supplementation against the control group. The same observation was noticed for haemoglobin, creatinine and PCV. With regard to blood urea there is a clear difference in urea values, G.A. lowering levels of blood urea for the groups 1, 2 and 3 when compared with group 4 which is control. The physical form of the ration showed no statistical differences between all treatments and all groups, but only slight variations within the groups. Only variation was noticed in the feed intake and the body weight gain. The corresponding carcasses were analyzed for standard chemical analysis parameters for meat. An in vitro experiment was conducted in the laboratory in which G.A. was added to rumen liquor at conc. of 0%, 5%, 10%, 20% and 30% respectively for the determination of liberated gases of fermentation, ammonia and volatile fatty acids. The group with 30% G.A. supplementation showed highly significant increase of ammonia and volatile fatty acids levels when compared with the control group.
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Keywords
Oxidase test,Blood ,Goats,Sheep,Microbial
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