Prevalence Of Avian Influenza Virus Type A Antibodies In Khartoum State And Study Of Three Serodiagnosis Techiniques

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Hassan, Alia
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The objective of the study was to investigate the prevalence of AIV antibody Ab in Khartoum state, and Compare between serological tests; AGID, HI and ELISA. Two hundred and fifty eight blood samples were collected in 2006 from twelve farms in Khartoum state and subjected to serologic screening for type-specific antibodies to type A influenza viruses, there was serologic evidence of past infection with AI virus type A in some flocks. Agar gel immunodiffusion (AGID) and an enzyme-linked immunosorbent assay (ELISA) tests were used for detecting Ab against type A avian influenza (AI) virus. Hemagglutination-inhibition (HI) test was used for subtyping of the corresponding antigen. The sensitivity and specificity of the AI-ELISA was compared in the various groups with those of the AGID and the hemagglutination-inhibition (HI) test; prapered hyperimmune sera and field sera. During the course of preparation of hyperimmune sera (0-80 days) of specific-antibodies-negative (SAN) chickens with AI subtypes A/Mallard/Neth/12100 (H7N3) and Duck/Sing/645.3/97/128 (H5N1), the AGID test was able to detect type A specific Ab as early as 14 days postinoculation(PI). Similarly, the AI-ELISA detected AI Ab and measured rising levels of Ab through 66 days PI, at which time the chickens were re-exposed to AI virus at 21 days post primary inoculation with the same AI subtype. HI detected low levels of AI Ab at 14 days PI. Following a secondary infection, HI measured rising levels of AI-specific Abs (35-80 days PI).