Studies on fowl spirochetosis in Khartoum State
Studies on fowl spirochetosis in Khartoum State
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Date
2015-04-12
Authors
Mohammed, Iman
Journal Title
Journal ISSN
Volume Title
Publisher
UOFK
Abstract
Spirochetosis was reported in different poultry farms in Khartoum
state. Clinical disease was recorded among different ages and breeds.
Based on morphology of the organism, presence of argas persicus clinical
signs and post mortem lesions in inoculated chicken, the organism was
confirmed as Borrelia anserina.
The disease was studied in three different age groups of chicken
(Chick, grower and adult) using four field isolates .Short incubation period
(2-3) days, severe clinical sings, extensive short duration of spirochete 3-4
day and enlargement and mottling of spleen were shown
in older birds. Mild clinical signs, extensive prolong spirochetemia 10-15
days, slight enlargement of the spleen were the main features of the disease
in chicks.
Histopathological examination of organs of infected birds showed
difference between the isolates .Two isolates showed severe changes and
damage of the spleen.
Disease in local breeds and pigeon was also studied.
A trail was carried out to isolate the causative agent in bacteriological
media but the organism failed to grew albeit that the organism was isolated
in embryonated eggs via different routes successfully.
Serological studies showed that Agglutination and immobilization tests
were both comparable in detecting Borrelia anserina antibodies. Antigenic
and pathogenic study of the 4 field isolate revealed that two isolates
(namely:Butri and Soba1) were more pathogenic.
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Cross protection test between the field isolates showed that the immune
response induced by infection with two of the isolate will protect against
challenge by the four isolate studied.
The effect of storage condition and duration on viability and infectivity of
Borrelia anserina was also investigated.
In other part of the study, molecular characterization of Borrelia
anserina was investigated. After extraction of DNA and amplification no
results were obtained.
Description
Keywords
DNA,Borrelia,birds,morphology,chicken