Local and systemic immune response of a combined anthrax and sheep pox vaccine
Local and systemic immune response of a combined anthrax and sheep pox vaccine
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Date
2005
Authors
MOHAMED, MOAWIA
MOHAMED, ABDELRAHIM
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Abstract
This study wascarried out to produce a combine anthrax and sheep pox
vaccine and to compare its immune response with each of the single sheep
pox and anthrax vaccine. Anthrax vaccine was produced in Roux flasks as a
live spore vaccine, while sheep pox vaccine (SPV) was produced on lamb
testicle (LT) monolayers. The combined vaccine was made from mechanical
mixture of Bacillus anthracis (Sterne strain) live spores and the concentrated
sheep pox vaccine, the mixture was lyophilized in 5 ml vials. The dose of
each component of the final product was 1.0x 109 live spores/ml and 2.5log10
TCID50/ml of anthrax and sheep pox vaccine respectively.
The potency of the combined vaccine was done by counting the number
of the viable spores and by titration of sheep pox virus on sheep flank
region. The viable spores count was found unchanged at the time
vaccination (6 months of preservation at – 200 C, the virus produced nodular
lesions on sheep flank when given intradermally.
When the end point titration of sheep pox virus was conducted on flank
region, the number of positive percentage was found 301and 277 for nonvaccinated
control, 84 and 25 for sheep vaccinated with sheep pox vaccine
and 50 and 54 for sheep vaccinated with the combined vaccine.
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Keywords
Immunity,Manufacturing and control requirements