Vaccine Trails Against Bovine Farcy
Vaccine Trails Against Bovine Farcy
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Date
2015-04-01
Authors
Mukhtar, Eiman Abd Elrahim Mohamed
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Journal ISSN
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Publisher
University of Khartoum
Abstract
The present study was designed to develop and evaluate a live vaccine
against bovine farcy. Mycobacterium farcinogenes (M. farcinogenes) strain
A20, was passaged in a set of guinea pigs. The attenuated strain produced no
lesions neither at the site of inoculation nor in any other organ of the tested
guinea pigs. The organism, which was reisolated from the guinea pigs, was
labelled as M. farcinogenes A24.
Another experiment was carried out in guinea pigs to determine the
degree of protection following the vaccination with M. farcinogenes A24.
Guinea pigs were divided into two groups. Vaccinated and non-vaccinated
groups. At the end of the 9th week, the challenge infection was performed
using a freshly isolated M. farcinogenes SD117, which was isolated from a
cow with bovine farcy. Ninety two percent (92%) of guinea pigs from
vaccinated group showed no nodular lesions, whereas all the non-vaccinated
guinea pigs showed visible nodular lesions.
Eleven calves were used for vaccine trials in bovine. The calves were
divided into three groups: vaccinated group (n=8), non-vaccinated group
(n=2) and control group (n=1). Seventy five percent (75%) of the vaccinated
calves showed no lesions following the challenge infection while all of nonvaccinated
calves showed the nodular lesions at site of inoculation.
ELISA readings among the vaccinated and non-vaccinated guinea pigs
showed that the mean optical densities at 450 nm were 0.15 and 0.05,
respectively.
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Keywords
Vaccine Trails;Bovine Farcy;Patholog; Mycobacteri