Bacteria Isolated From Sunff (Nicotiana rustica) Before and after Fermentation
Bacteria Isolated From Sunff (Nicotiana rustica) Before and after Fermentation
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Date
2005-8
Authors
Awatif Awad
Journal Title
Journal ISSN
Volume Title
Publisher
University of Khartoum
Abstract
The present study was planned to isolate and identify the types
of bacteria present in toombak .Sixty six samples of moist and dry
snuff were collected from different toombak shops in Khartoum,
Omdurman, and Khartoum north. The samples consisted of fifty moist
samples and sixteen dry samples.
The samples were subjected to bacteriological examination to
investigate aerobic and anaerobic bacteria.
No bacterial growth was obtained when the samples were
cultured anaerobically, while 195 bacterial isolates were obtained
aerobically from 66 toombak samples. 193 isolates were Gram positive
bacteria predominantly thirty isolates (15,5%) were Bacillus mycoides
,39 isolates (20,2%) were B. amyloliquefaciens,37 isolates (19,2%)
were B. subtilis,12 isolates (6,2%) were B .cereus,17 isolats (8,8%)
were B. thuingiensis,16 isolates (8,2%) were B. sphaericus, 14 isolates
(7,3%) were B .megaterium, 9 isolates (4,7%) B. coagulans, 9 isolates
(4, 7%) were B. licheniformis, nine isolates (4, 7%) were B. brevis, two
isolates (1, 0%) were B. alvei and one isolate (5%) was B.
pantothenticus.
While the total of Gram-negative bacteria was, only two isolates
belonged to Providencia.rettregi species. In this study, we measured
the level of pH and the redox potential of moist, dry and natron and
redox potential after addition of cystein hydrochloride to moist snuff
solution was also measured which became more reduced.
Description
A thesis submitted to Graduate College University of Khartoum in partial
fulfillment of the requirements of the Degree of Master of Veterinary
Science in Microbiology
Keywords
Fermentation
Bacteria Isolated
Sunff
Chemical compassion
tobacco
Microbiology
Veterinary
Science
Prevalence of smokeless-tobacco in the world;Processingof toombak;Contamination
Citation
Bacteria Isolated From Sunff (Nicotiana rustica) Before and after Fermentation / Awatif Awad .- Khartoum : University of Khartoum, 2005. - 133p. : illus., M.Sc.
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