Bacteria Isolated From Sunff (Nicotiana rustica) Before and after Fermentation

Abstract

The present study was planned to isolate and identify the types of bacteria present in toombak .Sixty six samples of moist and dry snuff were collected from different toombak shops in Khartoum, Omdurman, and Khartoum north. The samples consisted of fifty moist samples and sixteen dry samples. The samples were subjected to bacteriological examination to investigate aerobic and anaerobic bacteria. No bacterial growth was obtained when the samples were cultured anaerobically, while 195 bacterial isolates were obtained aerobically from 66 toombak samples. 193 isolates were Gram positive bacteria predominantly thirty isolates (15,5%) were Bacillus mycoides ,39 isolates (20,2%) were B. amyloliquefaciens,37 isolates (19,2%) were B. subtilis,12 isolates (6,2%) were B .cereus,17 isolats (8,8%) were B. thuingiensis,16 isolates (8,2%) were B. sphaericus, 14 isolates (7,3%) were B .megaterium, 9 isolates (4,7%) B. coagulans, 9 isolates (4, 7%) were B. licheniformis, nine isolates (4, 7%) were B. brevis, two isolates (1, 0%) were B. alvei and one isolate (5%) was B. pantothenticus. While the total of Gram-negative bacteria was, only two isolates belonged to Providencia.rettregi species. In this study, we measured the level of pH and the redox potential of moist, dry and natron and redox potential after addition of cystein hydrochloride to moist snuff solution was also measured which became more reduced.