Pharmacological Studies on three Sudanese Medicinal Plants Possessing Antimicrobial Properties
Pharmacological Studies on three Sudanese Medicinal Plants Possessing Antimicrobial Properties
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Date
2015-04-03
Authors
Mohamed, Mohana
Journal Title
Journal ISSN
Volume Title
Publisher
UOFK
Abstract
In order to verify or refute the folklorically claimed benefits of some
Sudanese plants possessing antimicrobialactivity and to detect any side
effects that may be associated with their use, the methanol extract of three
plants were studied on selected isolated tissue preparations.
The methanol extract of Loranthus acaciae (Zucc.) leaves exhibited
a dose dependent non-adrenergic inhibition of the isolated rabbit jejunum
preparation, since it was not blocked neither by propranolol (Non
selective β–blocker) nor tolazoline (Non-selective αblocker). The extract
was devoid of any activity on guinea pig ileum preparation and couldn’t
antagonize the Histamine (50ng/ml) induced contraction of the tissue.
Prior administration of the extract (2mg/ml) markedly potentiated the
Acetylcholine (1µg/ml) contracture on Toadrectus abdominis muscle
preparation, while the extract alone was without activity on the
preparation .
In a dose of 4mg/ml the extract effectively reversed d-tubocurarine
(20µg/ml) blockade of Acetylcholine (1µg/ml) contracture on toad rectus
abdominis muscle preparation in a manner comparable with that
produced by the anticholinestrase Neostigmine (40µg/ml). On both
isolated rabbit heart and guinea pig atrium preparations the extract
showed a dose dependent inhibition of the force of contraction but
without effect on the rate of contraction, its action on the atrium was not
affected by prior administration of Atropine sulphate (50µg/ml). Prior
administration of the extract (2mg/ml) to the cardiac muscle obviously
potentiated acetylcholine (2µg/ml) inhibition of the force of contraction.
The methanol extract ofNymphaea lotus (L.) showed a dose
dependent (1-4mg/ml) stimulation of the isolated rabbit jejunum
preparation, this stimulation was refractory to atropine sulphate (2µg/ml)
antagonistic property, but was blocked by Cyproheptadine (40µg/ml) (a
non selective 5HT blocker). The extract(2mg/ml) exhibited a contraction
of the guinea pig ileum preparation comparable with that of Histamine
(10ng/ml) but refractory to the antagonistic action of the antihistamine
Chlorpheneramine
maleate(40ng/ml),on the other hand the extract(2mg/ml) induced
contraction as well as 5Hydroxytreptamine were completely blocked by
Cyproheptadine (20ng/ml). On rat fundus strip preparation both
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5Hydroxytreptamine (25ng/ml) and Nymphea lotus methanol extract
(2mg/ml) showed a prominent contraction that was completely blocked
by Cyproheptadine. The extract (2mg/ml)neither blocked nor potentiated
the Acetylcholine (1µg/ml) induced contracture of the toad rectus
abdominis muscle preparation .the extract per se showed no activity.
The methanol extract of Anogeissus leiocarpus (DC.) showed a dose
dependent inhibition of the isolatedrabbit jejunum preparation. This
relaxant activity was not blocked by Tolazoline (40µg/ml) while
propranolol (4µg/ml) completely blocked the inhibition. The extract
(2mg/ml) per se was without effecton both isolated guinea pig ileum
preparation and isolated toad rectusabdominis muscle preparation. The
extract neither antagonized nor potentiated the carbachol (1µg/ml)
contraction on the guinea pig ileumpreparation and Acetylcholine
(1µg/ml) contracture on toad rectus abdominis muscle preparation.
On guinea pig atrium preparation the extract (2mg/ml) decreased
markedly the frequency of contraction and was devoid of any activity on
the force of contraction. The extract (4mg/ml) neither had any activity on
isolated guinea pig tracheal preparation nor affected histamine
(400ng/ml) induced contraction of the preparation. On rat uterus
preparation the extract (2mg/ml) showed relaxant activity that blocked
the Acetylcholine (1µg/ml) contraction of the preparation, prior
administration of propranolol (80µg/ml) was without effect.
Description
Keywords
Pharmacological Studies,Sudanese Medicinal Plants,Antimicrobial Properties