Studies on the Sudanese Honeybee Venom Extraction, Analysis and Antiparasitic Effect on Leishmania spp)

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Date
2015-05-10
Authors
Elhag, Mageeda Siddig
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Publisher
UOFK
Abstract
This study was carried out in the Apiaries of both the Faculty of Agriculture, University of Khartoum in Shambat and El-Khalia Investment Company in Al-Faki Hashim during the periods between 1999 and 2001. An electric device was designed for exciting honeybee workers to produce venom in a powder form. The study emphasized effects of some factors viz. voltage, period of exposure to electrical shocks, and interval between electrical shocks. The largest amount (40.04 mg/colony) was produced by 24 volts for 30 minutes every 18 days. Chemical analysis was carried out on the product and the medical properties were tested in vitro on Leishmania spp. The major constituent of bee venom was the protein giving 75 mg/dl, followed by urea (2.34 mg/dl) and uric acid (0.08 mg/dl). As well as 1.21 phosphorus, 0.91 calcium, 0.8 magnesium and 0.036 iron (mg/dl). In viro studies showed that honey bee venom has lethal effects on both species, Leishmania donovani and L. major depending on its concentration, the highest effect was produced by the highest concentration used (1 mg of venom) giving initial 90% mortality.
Description
2003
Keywords
Sudanese Honeybee , Leishmania spp
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