Detection of Orthopoxvirus Antibodies in Sera of Some Domestic Species in the Sudan
Detection of Orthopoxvirus Antibodies in Sera of Some Domestic Species in the Sudan
No Thumbnail Available
Date
2015-04-12
Authors
Tag Elsir, Siham
Journal Title
Journal ISSN
Volume Title
Publisher
University of Khartoum
Abstract
This study aimed to know the biological properties of Vaccinia
virus in the Chorioallantoic membrane of the chick embryo and cell
culture. Also to study the epidemiology of orthopox virus in sheep, cattle
and camels in the Sudan as the first study from this type.
Vaccinia virus strain is used in this study for propagation in
the Chorioallantoic Membrance (CAM) of embryonated eggs. Lesions
were seen as small pock ranging from 0.1-0.2 mm in diameter, round,
opaque and white in colour. The histopathology of these lesions showed
foci of epithelial thickening, swollen cell with pale cytoplasm,
eosinophilic inclusion bodies and infiltration of mononuclear cells.
When propagated in Vero cells, this virus gave clear Cytopathic
effect characterized by rounding of the cells, plaque formation, syncytia
and detachment of cells from flask surface.
Agar Gel Immuno Diffusion Test (AGID) was used in this study
to detect orthopoxviruses antibodies in serum samples collected from
cows, sheep and camels from different areas of Sudan.
The study revealed that orthopoxvirus antibodies are present in
these animals with varying range. The seroprevalence was 35.7% in
cattle, 77.9% in sheep and 53.5 % in camel. That means antibodies
against orthopox viruses are widely distributed in sheep, cattle and camel
in the Sudan.
Description
53 Pages
Keywords
Orthopoxvirus, Antibodies,Sera, Domestic Species ,Sudan