Susceptibility of Culex quinquefasciatus

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Omair Osman Wahbi, KhatirProfessor. El Amin El Rayah
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This study aimed to compare the susceptibility of culex quinquefasciatus to Organochlorine (DDT%4), Organophosphates (Malathion 5%), Carbamates (Bendiocarb 0.1%) and Pyrethroids (Deltamethrin0.05%). Larvae and pupae were collected from breeding sites from Nabta residential area and three farms from Shambat; the standard scope method was used for collection. In the laboratory collected larvae and pupae were reared to adulthood, whereby 1-3 days old ) unfed female (were used for susceptibility test. Twenty five female were exposed for each insecticide at four replicate in addition to control were performed using the World Health Organization test kits (protocol 1998). These contain exposure /holding tubes, copper and silver rings, insecticide –impregnated filter papers, oil – impregnated control papers and sucking tubes. Susceptibility was recorded after 10, 15, 20, 30, 40, 50 and 60 minutes, the test chamber inverted to exposed the female to oil treated filter paper, then susceptibility was recoded after 24 hours. The susceptibility of each pesticide was compared between the two collection sites using t-test. Within each site all pesticide were compare using one way analysis of variance. The results showed significant difference (p<0.05) in susceptibility to DDT and Bendiocarb between two areas, however there are no significant difference (p>0.05) in susceptibility to Detamethrin and malathion. Analysis of variance indicate that there are significant difference in susceptibility to Deltmithrin and other insecticide were used in Nabta ,however there are significant difference between DDT and Deltamethrincomparing with Malathion and Bendiocarb in Shambat farms. This study recommended that to reduced mosquitoes breeding site by covered Networks of draining waste water system (manholes) in Nabta and avoid flowing the water out of irrigation channels in Shambat farms.
Susceptibility of Culex quinquefasciatus