Effect of Unilateral Vasectomy on the Morphology, Morphometry and Histochemistry of Ram Testis

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Date
2015-06-22
Authors
Tahani Omer Abdel Gader El Amin, El Amin
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Publisher
UOFK
Abstract
The effect of unilateral vasectomy was studied in eleven adult desert rams over periods ranging from 2 to 16 weeks. Parameters investigated included testicular weight and size, histological and morphometric features and histochemical studies of both intact and vasectomized sides of the genital tract. The measurements carried out after all post operative periods, showed small testicular size. The weights of vasectomized testes also decreased and showed minimal values at two weeks after operation, amounting to about 69.85% of the contralateral control. Histometrical analysis of the vasectomized testes, showed a marked atrophy of the areas occupied by the seminiferous tubules. This was clearly observed in the testes of animals examined at two, twelve and sixteen weeks after operation. General histological observations showed degenerative changes only in the testes of animals examined at two weeks after operation, the epithelium was reduced to a single peripheral layer of spermatogonia. The testes of animals examined beyond ten weeks after vasectomy showed normal structure comparable to that of controls. The distinct alteration which occurred after ten weeks post operation was in the form of dense accumulation of collagenous fibers in the interstitial and peritubular tissue. A positive histochemical reaction for carbohydrates, lipids, alkaline phosphatase, steroid dehydrogenase and succinate dehydrogenase were demonstrated mainly in the seminiferous epithelium with a relatively weaker reaction in the interstitial tissue. Reactions of glycogen, alkaline phosphatase and steroid dehydrogenase were not affected by vasectomy. The PAS positive, diastase resistant materials, lipids and succinate dehydrogenase were clearly affected by vasectomy. The vasectomized testes showed thickening of PAS positive, diastase resistant materials in the boundary tissue of the seminiferous tubules, while the presence of glycogen particles was dependant on the spermatogenic cycle and not affected by vasectomy. Lipid droplets increased in amount and size after vasectomy. Alkaline phosphatase was demonstrated in the seminiferous tubules in both control and vasectomized testes. Positive reactions of Δ5 3ß hydroxysteriod dehydrogenase and 17ß - hydroxysteriod dehydrogenase were mainly depicted in the seminiferous tubules of control testes and similar reaction was also observed in the vasectomized testes, indicating that the steroid hormones which were synthesized by testicular tissue were not affected by vasectomy. Succinate delydrogenase was mainly demonstrated depicted in the seminiferous tubules of control testes while vasectomized testes showed an intense reaction in the interstitial tissue.
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Effect of Unilateral Vasectomy on the Morphology, Morphometry and Histochemistry of Ram Testis
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