Contagious Bovine Pleuropneumonia Immunity in Calves

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Date
2015-06-22
Authors
: Abdel Mutaal Abdalla Obied Elshalali, Elshalali
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Publisher
UOFK
Abstract
In this study, it was found that calves (3) inoculated by intratracheal endobronchial method with virulent strain of Mycoplasma mycoides showed arthritis with lameness. Pulmonary form of the disease was produced. On post mortem examination, no necropsy lesions were observed in lungs and other internal organs. M. mycoides was isolated from the synovial fluid of these calves. Zebu (4) and exotic breed calves (16) vaccinated with T1/44 broth culture vaccine were found solidly immune when challenged six months and nine months post-vaccination, respectively. The control (non-vaccinated) calves showed arthritis with lameness post-challenge. Using the complement fixation test and ELISA, it was found that the antibodies persisted in sera of vaccinated local and exotic calves for 3-4 months and 5-6 months, respectively. It was found that calves born recently from vaccinated dams developed antibodies one week post-parturition and reached its maximum after one month and then gradually declined. By the 6th month, 70% of the test calves sera were found negative. Based on these results it was found that six months was the suitable age to start vaccination of calves against contagious bovine pleuropneumonia (CBPP). The study was concentrated on the role of cell mediated immunity against CBPP. It was found that marked inhibition was given with the leukocyte cells from vaccinated calves when 20% concentration of Mycoplasma antigen was added to the medium. But with non-vaccinated calves no inhibition of migration in presence or absence of Mycoplasma antigen was observed. The bioreactor technology was introduced for the first time for production of CBPP vaccine. It was noticed that larger amounts of he vaccine with higher viable count 1010-12 CFU/ml was produced in a shorter time compared with the traditional method. The bioreactor method reduces the man-power, time, space and t]he bacterial and fungal contamination. Sterility, innocuity and safety tests carried out for some batches of vaccines showed that both T1/44 and T1/44/SR were equally immunogenic and safe for Sudanese calves. It was confirmed that ELISA was more sensitive than CFT in detecting antibodies against M. mycoides.
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Bovine Pleuropneumonia Immunity in Calves
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