Phytochemical Screening For Khellin In Some Khella Seeds (Ammi Visnaga And Ammi Majus) Cultivated In The Sudan

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Esmahan Al Tyib Hassan Abosalah
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University of Khartoum
Five samples of Ammi visnaga and Ammi majus seeds were collected from different sources in the Sudan. Phytochemical screening of extracts of A. visnaga and A. majus revealed the presence of various components such as saponin, Ttriterpens, Alkaloids, Flavoneids, Tannins and coumarins in addition to khellin. Extracts obtained from A. visnaga and A. majus seeds were distinguished by colour reaction where they gave faint yellowish green and dirty brownish green colour respectively. Extractions with chloroform and ethanol 60% for seeds of A. visnaga and A. majus samples were performed. Khellin was identified by thin layer chromatography. The identification was carried out by reaction colour, UV366 and Rf value were determined and khellin was identified by comparison against standards. Quantitative determination of khellin revealed variation in khellin content in the studied samples. It was found that A. visnaga collected from Shambat in 1993 and 1998 gave 0.627% and 1.892% khellin respectively, while A. majus seeds collected from Omdurman and Elobeid markets and from Shambat in 1993 did not contained khellin The study revealed that the extracts of all samples of A.visnaga under investigation contained variable amount of khellin and the highest content was found in A. Visnaga collected at Shambat in 1998.
A dissertation Submitted In Partial Fulfillment of the Requirements for the Degree of Master of Science in Food Science and Technology
Phytochemical Screening Khella Seeds Ammi visnaga Ammi majus Food Science And Technology University Of Khartoum
Esmahan Al Tyib Hassan Abosalah, Phytochemical Screening For Khellin In Some Khella Seeds (Ammi visnaga and Ammi majus) Cultivated In The Sudan. – Khartoum : University of Khartoum, 2004. - 69 P. : illus., 28 cm., M.Sc.