The Antiviral Effect Of Acacia Nilotica Extracts Against The Mesogenic Strain Of Newcastle Disease Virus
The Antiviral Effect Of Acacia Nilotica Extracts Against The Mesogenic Strain Of Newcastle Disease Virus
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Date
2015-04-12
Authors
Mahmoud, Amel
Journal Title
Journal ISSN
Volume Title
Publisher
University of Khartoum
Abstract
Methanol, chloroform and water extracts of Acacia niloticasub
species tomentosawere tested in-vitro for their potential antiviral activity
against the Komarov vaccine strain ofNewcastle disease virus (NDV). Sub
culturing the treated samples and heamagglutination test were used to
evaluate the reduction ofviability in the viral growth in presence and
absence of the effective extract. The noncytotoxicconcentration of each
extract was determined by the absence of the CPE. The inhibitory
concentration of the effective extract was measured. The tannin percentage
of the methanol and water extracts were evaluated by Prussian Blue assay.
The methanol extract of Acacia nilotica on vero cells showed non-
cytotoxic concentration of ≥40µg/ml. The methanol extract showed a
significant inhibitory effect against the tested virus at the concentration of 40
µg/ml. None of the other two extracts showed any inhibitory activity.
Subculturing of the treated samples of the methanol extract showed no CPE
compared to the control. The HA test showed zero titer of the tested samples
against 2 log 6 to the control.
The tannin percentage ofthe methanol extract were higher (1.6%)
than the water extract percentage (1.2%).
Description
Keywords
Castle Disease Virus,Mesogenic Strain,Acacia Nilotica