Effect of Vasectomy on the Morphology and Histochemistry of the Accessory Sex Glands of Male Rabbit

Abstract

Thirty two adult rabbit's local strains were applied unilateral and bilateral vasectomy. Morphological, histometrical, histochemical and ultrastructural studies have been carriedout on the seminal vesicles, prostate and bulbourethral glands of control and vasectomized animals. The specimens have been taken after slaughtered animals on days 15, 30, 60 and 112 after unilateral and bilateral vasectomy. The aim of the experiment was to study the effect of unilateral and bilateral vasectomy on the morphology, histometry, histochemistry and ultrastructure of the seminal vesicles, prostate and bulbourethral glands. The effect of unilateral and bilateral vasectomy, in these glands was gave significant changes on day 60 and reached a peak on day 112, compared to the control. The histological changes demonstrated the increase in connective tissue fibers and secretory productson day 60 after vasectomy, decrease in the size of lobule and drop in cell height and secretory products in day 112 after vasectomy. These changes were approved by histometric measurement. The histochemistry observation showing an increase in PAS- reaction in prostate gland, other what no changes in seminal vesicles and bulbourethral glands, acid phosphatase activity showing gradual intense and there were no changes in alkaline phosphatase in these glands. Ultrastrucural studies demonstratedvariation in nuclei shape, atrophy in the mitochondria and endoplasmic reticulum inseminal vesicles and prostate gland and proliferation of mitochondria in the bulbourthral glands, many electron dense bodies and large empty vacuoles.