Use of Antibiotic-Resistance Markers and Serological Techniques in Ecological Studies of Root-Nodule Bacteria

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Date
2004
Authors
Mahdi, A.A.
Diab, M.M.O.
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Publisher
Faculty of Agriculture, University of Khartoum, Shambat, Sudan
Abstract
Two field investigations and a pot trial involving three types of soil during the period 1999-2000 indicated the presence of adequate numbers of faba bean-nodulating bacteria in Shambat soil. Inoculation with an introduced or a local bacterial strain, or both, improved nodulation but not plant growth or seed yield. Determination of nodule occupancy by antibiotic-resistance markers or serological techniques revealed that carrier-formulated inoculants succeeded in outcompeting indigenous strains for nodulation in the field, while broth inoculants commanded 68.5% of nodulation in pots. The use of these techniques in ecological studies of root nodule bacteria is commended.
Description
Page(s): 12 (2), 173-184,10 Ref.
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