Comparative Study of Four Seroagglutiny Serum Tests for Brucillosis in Dairy Cattle in Port Sudan City, Sudan

Abstract

The objective of this work was to study the seroprevalence of brucellosis in dairy cattle in Port Sudan city, the Red Sea Sate, Sudan. Two hundred and fifteen blood samples were collected aseptically from three locations in the city bearing in mind the density of animals in each location. Sera were separated from clotted blood samples and tested by four screen tests: The modified Rose Bengal Plate Test, the Rose Bengal Plate Test, the Serum Agglutination Test and the competitive Enzyme-linked Immuosorbent Assay. The seroprevalence rate was 21% by modified Rose Bengal plate Test and 13.0% by Rose Bengal plate Test. The Serum Agglutination Test detected 93% of the 27 samples positive for the Rose Bengal Plate Test. The highest titre for SAT was 1488 IU/ml and the lowest was 20 IU/ml. Only 46% of 46 cattle found positive with the modified Rose Bengal plate Test were confirmed by the modified Rose Bengal plateTest prove positive by the competitive Enzyme-linked Immunosorbent Assay. The results showed that the modified Rose Bengal Plate Test was the most sensitive of the four followed by the Rose Bengal Plate Test but were less sensitive than the competitive Enzyme-linked Immunosorbent Assay. These findings are discussed with respect to improved diagnosis and control of brucellosis in animals and humans in Eastern Sudan with emphasis on urban areas.