Isolation, Characterization,and Determination Of Cytotoxic Effect Of Escherichia coli, Isolated From Poultry

Abstract

Sixty samples were collected from different organs of poultry, 16 of these samples were livers, 15 intestines, 7 lungs, 5 hearts, 5 spleens, 4 eye swabs, 3 ovaries, one pharynx, 2 kidneys, one brain and one embryo. Out of these samples 50 isolates were isolated and identified as Escherichia coli. All the isolates were negative to oxidase test, positive to catalase test, Voges. Proskaur negative, MR test positive, H2S negative and ONPG positive. All the isolates fermented glucose, maltose, sorbitol and raffinose. 84% of the isolates fermented sucrose, 88% were positive to indole test, 86% were negative to urease test and 78% of the isolates were molile. All the isolates were fermentative. Ten strains of E. coli were selected according to the differences in results of biochemical tests and investigated to determine whether they produce cytotoxic activity in Vero cells and Bovine kidney cells. All the isolates produced cytotoxic activity in bovine kidney cells although they did not produce cytotoxic effect in Vero cells.