Antibacterial effect of Nigella sativa L.Using the Conventional Bacteriological Methods and Polymerase Chain Reaction

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Osman, Imadeldin
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E. colistrains were detected in fresh fecal samples collected from the lower large intestine and cloaca in broilers. The birds were fed on different concentrations of Nigella sativa crushed seeds to demonstrate antibacterial effect of the seeds .The organism was identified using conventional bacteriological methods and the polymerase chain reaction (PCR).The fecal sample were subjected to total bacterial count and then ,the identity of the nucleic acid sequence of the organism was confirmed using polymerase chain reaction-based detection assay (PCR). In the PCR assay, a pair of primers derived from uidA gene of E. coli, encoding glucuronidase specific for E. coliwas used. Conventional isolation and identification is the most accurate method for detection of an active (intact) organism in fecal samples. However, this method is tedious, laborious and time consuming.The results of this study confirmed the presence of E.colistrains in fecal samples collected from all broilers groups .No clear effect of N.sativaas antibacterial agent was observed. In this work it is indicated that PCR should be used as a routine diagnostic technique for rapid detection of E.coliin clinical samples. It is worth mentioning that the described PCR-based provides rapid, sensitive and specific detection of both pathogenic and non-pathogenic strains of E. coli. Therefore, the diagnostic potentialof a positive PCR result has to be interpreted with caution in light of the presence or absence of pathologic strains of E. coli.
56 Pages
Nigella sativa, Conventional,Bacteriological Methods,Polymerase Chain Reaction;Primer design;Housing Management;Bacteriological Investigation;Bacteriological Media;Isolation Purification;bacterial count