HLA Frequency of HLA-DRB1&HLA-DQB1 alleles in Mycetoma patients

Abstract

Mycetoma (maduromycosis), True fungi-eumycetoma is the common cause of black grain eumycetoma in Sudan. It includes madurella mycetomatis-cause about 70% - 75% of all mycetoma cases in Sudan. The present study, which is the first molecular report on the association of HLA class-II alleles with Mycetoma infection in Sudanese patients. The objective of this study was to determine the frequency of the different alleles of HLA class II (DRB1, DQA1 and DQB1) in eumycetoma infected patients of Sudanese genetic backgrounds in an effort to determine possible association between the prevalence of certain alleles that could be associated with the occurrance eumycetoma infection. A total of 53 eumycetoma patients and 55 healthy control individuals were selected to determine the frequency of HLA-DRB1& HLA-DQB1. Both HLA-DQB1 and HLA-DRB1 genotyping were performed using PCR and sequence-specific (SSP) reverse hybridization oligonucleotide probe and analyzed with the Lambda Key Typing System and Lambda software. HLA-DRB1*13 and HLA-DQB1*06 were significant present in eumycetoma patients respectively (OR=2.543& chi-Square test =0.028) (OR=4.552& chi-Square test =0.000), while HLA-DRB1*11 and DQB1*01 (OR=0.313& chi-Square test =0.016) (chi-Square test =0.001) were less prevalent in eumycetoma patients.