Methicillin resistance among Staphylococcus intermedius isolated from routine veterinary microbiological diagnostics

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ElYas, Ibtisam
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The present study was designed to investigate methicillin resistant S. intermedius (MRSI) that had been isolated during routine veterinary microbiological diagnostics between Septem-ber 2004 and September 2005 phenotypically and genotypically for resistance properties. The species identity of the MRSI and selected methicillin sensitive S. intermedius (MSSI), used for control purposes, were confirmed by conventional methods and by PCR mediated amplification of S. intermedius specific segments of thermonuclease encoding gene nuc. All 33 MRSI, representing approximately 4 % of the S. intermedius isolated during the study period were oxacillin/methicillin and penicillin resistant using disk diffusion tests and reacted positively by multiplex PCR detection of the resistance genes mecA and b/aZ. The MRSI were also resistant to 7 up to 18 individual antibiotics indicating their multidrug resistancy. The MRSI of the present study were additionally examined by commercial MRSA-detection tests such as latex agglutination for detection PBP2a and growth on CHROMagar MRSA and oxacillin resistant screening agar base, partially indicating the usefulness of these screening tests also for S. intermedius. The MRSI were also studied for the distribution of virulence genes, namely siet and se-int encoding S. intermedius specific exfoliative toxin and entero-toxin respectively, yielding positive results for more than 57 % of the strains investigated.
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