C- banding in the Karyotype of Two Pennisetum Species

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Salih, M.A.M.
Eltigani, S
Abdalla, A.H
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Faculty of Agriculture, University of Khartoum, Shambat, Sudan
Kartyotypic analysis, using the C-banding technique, was carried out to study the morphology of the chromosome complements of Pennisetum glaucum and Pennisetum violaceum. In addition, the number of B- chromosomes was determined in the cells of the root tips of both species. Chromosome length ranged from 3.92 to 6.00 μm in P. glaucum, whereas in P. violaceum it ranged from 3.00 to 4. 50 μm. The haploid chromosome complement of P. glaucum consisted of two metacentric, three slightly sub- metacentric, one sub- metacentric and one highly sub-metacentric, while in P. vilaceum it consisted of two metacentric, two slightly sub-metacentric and two sub-metacentric and one highly sub-metacentric. Estimates of symmetry indices were 65.3 and 66.7, and of total formula were 44.29% and 43.17% for P. glaucum and P. violaceum, respectively, indicating the symmetry of the chromosome complements of both species. C-banding revealed the presence of a telomeric heterochromatin band on each arm of each chromosome in both species. The amount of the telomeric heterochromatin represented 24.2 and 31.5% of the total genomic chromatin in P. glaucum and P. violaceum, respectively. In P. glaucum, the number of B-chromosomes was between one and three with a mean of 1.75 per cell, whereas in P. violaceum it ranged from one to five with a mean of 2.45 per cell. The implications of the similarities and the differences on the gene flow between these species are discussed
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