Micropropagation of Jasmine (Jasminum azoricum L.) Using Tissue Culture Techniques

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Layous., L.N
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Faculty of Agriculture, University of Khartoum, Shambat, Sudan
A rapid and improved procedure of micropropagation of jasmine (Jasminum azoricum L.) was studied. Different types of tissue explants were cultured on MS salt mix containing different concentrations of naphthalene acetic acid (NAA), benzyl- aminopurine (BAP) and kinetin. The explants culture was conducted at different times in order to assess time effects on bud proliferation, elongation and root formation and to obtain proper clones after acclimatization. The results showed that kinetin (0.5 mg/L) on the MS medium gave the best result with regard to bud proliferation and multiplication. However, addition of NAA of different concentrations was inhibitory. The response of base and intermediate cuttings was better than terminal ones. The best time for bud proliferation in primary explants culture on MS medium supplemented with 0.5 mg/L kinetin was February and for bud multiplication February and March. Using 1.5 mg/L IBA in in vitro rooting medium, gave 97 % rooted plantlets, while ex vitro rooting of plantlets on agricultural media after soaking their bases in a solution containing 20 mg/L IBA increased rooting percentage up to 99%. In addition, the ex vitro rooting resulted in successful hardening of 94% of the plantlets and reduced time, costs and efforts required for rooting and hardening.
Page(s):12 (2), 293-308, 25 Ref.