Detection of Common Chromosomal Translocations in Acute Lymphoblastic Leukemia in Sudanese children
Detection of Common Chromosomal Translocations in Acute Lymphoblastic Leukemia in Sudanese children
No Thumbnail Available
Date
2006-05
Authors
Mohammed, Sara
E. Ibrahim, Muntaser
Journal Title
Journal ISSN
Volume Title
Publisher
University of Khartoum
Abstract
This study aims initially to introduce molecular technique, which are reliable and
sensitive in detecting cells beyond the detection level of conventional
cytomorphology, to be applied in the routine diagnosis of Leukemia in Sudan. And to
detect roughly the frequency chromosomal aberrations in acute lymphoblastc
leukemia in Sudanese children.
RNA was extracted from leukemia patients’ blood, using Trizol method. Detection of
leukemia specific markers (fusion gene) was carried after converting the RNA into
cDNA, using specific primers for the fusions. We selected the five common fusion
genes occur in Acute Lymphoblastic Leukemia, the ABL-BCR both the p210 and
p190, TEL-AML, E2A-PBX, and the MLL-AF4 fusion genes. To confirm the result
Dot blot hybridization technique was done using BCR-ABL-p210 probe, which gave
a strong signal with the BCR-ABL-p210 positive sample.
The technique is shown to be easy to conduct, reliable and specific.
Three fusion genes were detected, TEL-AML, E2A-PBX, ABL-BCR and their
frequency was 36%, 4%, 4% respectively, and were detected at various stages of
diagnosis and treatment in some patients.
The identification of patients with positive fusion genes at remission, highlight the
potential of using this technique for diagnosis of patients with minimum residual
disease (MRD). In order to achieve well in leukemia treatment, and to maximum
benefit of this technique, follow up of patients should be done according to the
treatment Regimen and in good collaboration between the lab and clinic
Description
Keywords
Acute lymphoblastic leukaemia (ALL),Leukemia,Molecular Genetics of Childhood Cancer,Hematopoiesis