Studies On Infectious Bovine Rhinotracheitis Virus (Bovine Herpesvirus-1) In Sudan
Studies On Infectious Bovine Rhinotracheitis Virus (Bovine Herpesvirus-1) In Sudan
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Date
2015-04-01
Authors
Mohamed, Amira
Journal Title
Journal ISSN
Volume Title
Publisher
UOFK
Abstract
In the present study 78 swab samples were collected
for isolation of infectious bovine rhinotracheitis virus (IBR)
from cases suffering from infertility, nasal discharges, vaginal
discharges, lacrimation and abortion. The IBR virus was
isolated from different states of the Sudan; five cases have
typical signs of the disease including two from Nyala, two
from Atbara and one from West Kordofan. Out of these 78
cattle, 24 serum samples were found to have neutralizing
antibodies to IBR virus when tested by SNT, PHA and
ELISA, which confirmed the presence of the disease.
Three IBR strains (Los Angeles, Kenya and South
Africa) were propagated in BKC and other cell culture
including LKC, LTC, VERO and BKCL to determine the
biological infectivity to these strains in different tissue culture
cells. All viruses showed typical CPE to IBR within 24 hours
post inoculation. The BKCL and LKC were the best cells in
that they produced CPE, which covered 80-90% of cell sheet
within 48 hours.
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Hyperimmune serum was prepared in two rabbits and
the serum was collected to be tested by neutralization and
passive haemagglutination test for the presence of antibodies.
For serosurveillance purposes 856 serum samples were
collected from different states of the Sudan. The overall
percentage of positive samples was 47.43% (406 samples)
tested by PHA. Of 273 samples tested by ELISA 141 were
positive (51.65%), whereas 69 out of 94 samples tested by
SNT (73%) were positive. The prevalence was found high in
states including Jongeli, West and North Kordofan.
In comparison between the three serological tests,
SNT is recommended to be the technique of choice followed by
ELISA, while PHA is a good technique to define the disease.
Description
Keywords
Infectious Bovine,Rhinotracheitis,Virus,Sudan