A Note on Extending the Host Specificity of Bradyrhizobium japonicum and Rhizobium leguminosarum through Inter-generic Protoplast Fusion

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Date
2002
Authors
El Gaali, E.
Kitamoto, Y.
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Faculty of Agriculture, University of Khartoum, Shambat, Sudan
Abstract
In an attempt to extend the rhizobial host specificity, intergeneric protast fusion between autotroplic mutants of Rhizobium leguminosarum and Bradyrhizobium japonicum, which inoculate pea and soybean, respectively, were examined. Six stable fusion products (B1-B6) were selected and tested on the two host plants. Similar to R. leguminosarum, the fusion products B3, B5 and B6 induced nodules on the roots of the pea plants. However, B6 also induced nodules on the roots of soybean plants, indicating that this fusion product exhibited an extended host specificity twards the two species. B6, also, showed a substantial increase in the nitrogenase activity in both pea and soybean.
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Page(s): 10 (1),131-135,5 Ref.
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