University of Khartoum

Evolutionary conservation of RNA editing in the genus Leishmania

Show simple item record Ibrahim, Muntaser E. Abdel Hamid, Muzamil M. Bereir, Rihab E. Giha, Rania S.
dc.contributor.editor Abdel Hamid, Muzamil M. en_US
dc.contributor.other Molecular Biology en_US 2008 2015-11-12T05:15:18Z 2015-11-12T05:15:18Z 2015-11-12 2015
dc.description.abstract RNA editing in kinetoplastids is the process by which vital genetic informations are restored through insertion and deletion of uridine residues in coding sequences, particularly those of the mitochondrial pre-mRNA. Mammalian infecting Leishmania were not analyzed before for the presence of RNA editing to establish whether the mechanism is still in use in higher lineages of the genus. The Cytochrome Oxidase gene of Leishmania tarentolae is known to be edited at its 30 end with gRNA encoded in the region immediately downstream. We sequenced DNA and cDNA of the COII gene of Leishmania donovani and compared those to Leishmania tarentolae sequences from the database. The results reveal an insertion of uridines in a manner identical to L. tarentolae, leading to restoration of the amino acid sequence with relative conservation of the gRNA region. We conclude that RNA editing as a posttranscriptional mechanism is still conserved within higher evolutionary lineages of the genus Leishmania. # 2008 Published by Elsevier B.V. en_US
dc.publisher university of khartoum en_US
dc.subject RNA editing; Leishmania donovani; Leishmania major; gRNA en_US
dc.title Evolutionary conservation of RNA editing in the genus Leishmania en_US
dc.type Other en_US
dc.Faculty Endemic Diseases en_US

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