Diagnosis of visceral leishmaniasis by the polymerase chain reaction using blood, bone marrow and lymph node samples from patients from the Sudan

dc.FacultyAfrican and Asian Studiesen_US
dc.contributor.authorElhassan, Ahmed M.
dc.contributor.authorKhalil, Eltahir Awad G.
dc.date1997
dc.date.accessioned2015-12-01T09:05:11Z
dc.date.available2015-12-01T09:05:11Z
dc.date.issued2015-12-01
dc.date.submitted2015
dc.description5 Pages
dc.description.abstractWe have evaluated the sensitivity of the polymerase chain reaction (PCR) as a diagnostic tool for Leishmania donouani using blood, bone marrow and lymph node samples from Sudanese patients with a confirmed infection. Forty patients were diagnosed by microscopic examination of bone marrow or lymph node samples. The PCR was able to detect parasite DNA in 3 7 out of 40 blood samples. In bone marrow and lymph node samples, the PCR was able to detect parasite DNA in all 7 and 6 samples, respectively. We suggest that the PCR should be considered as a valuable and sensitive tool for the diagnosis of 1.. donouani infection. However, if PCR diagnosis is to supplement or even replace microscopic diagnosis in developing countries, a large number of patients with no apparent signs of infection and patients with other diseases have to he tested in order to evaluate its true potential.en_US
dc.identifier.urihttp://khartoumspace.uofk.edu/123456789/17382
dc.publisheruniversity of khartoumen_US
dc.subjectvisceral leishmaniasis, diagnosis, PCRen_US
dc.titleDiagnosis of visceral leishmaniasis by the polymerase chain reaction using blood, bone marrow and lymph node samples from patients from the Sudanen_US
dc.typePublicationen_US

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