Evaluation of the Polymerase Chain Reaction in the Diagnosis of Cutaneous Leishmaniasis due to Leishmania major: A comparison with direct Microscopy of Smears and Sections from Lesions

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dc.FacultyEndemic Diseasesen_US
dc.contributor.authorElhassan, Ahmed M.
dc.date1996
dc.date.accessioned2015-11-29T11:05:14Z
dc.date.available2015-11-29T11:05:14Z
dc.date.issued2015-11-29
dc.date.submitted2015
dc.description.abstractWe have compared the sensitivity of the polymerase chain reaction (PCR) as a diagnostic tool against conventional microscopical diagnostic techniques in patients with cutaneous leishmaniasis from the Sudan. Twenty-eight patients were diagnosed according to clinical criteria followed by microscopical examination of histological sections and slit or impression smears. The PCR had a sensitivity of 86% when used alone, and 93% when combined with Southern blotting. In contrast, microscopy of histological sections had a sensitivity of 76% and slit and impression smears of only 55% and 48%, respectively. The PCR should be considered as a valuable and sensitive diagnostic tool in the diagnosis of cutaneous leishmaniasis; it has the added advantage of identification of the species of Leishmania causing the lesion.en_US
dc.identifier.urihttp://khartoumspace.uofk.edu/123456789/17351
dc.language.isoenen_US
dc.publisherOxford Journalsen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectdiagnosisen_US
dc.subjectLeishmania majoren_US
dc.subjectcutaneous leishmaniasisen_US
dc.subjectleishmaniasisen_US
dc.titleEvaluation of the Polymerase Chain Reaction in the Diagnosis of Cutaneous Leishmaniasis due to Leishmania major: A comparison with direct Microscopy of Smears and Sections from Lesionsen_US
dc.typePublicationen_US

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