Detection and characterization of Leishmania in tissues of patients with post kala-azar dermal leishmaniasis using a specific monoclonal antibody
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Date
2015-12-01
Authors
Elhassan, Ahmed M.
A, Kharazmi
Journal Title
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Publisher
university of khartoum
Abstract
Sections from skin lesions and draining lymph nodes of patients with post kala-azar dermal leishmaniasis were examined using an immunoperoxidase method and a monoclonal antibody directed against Leishmania donovani. Parasites were detected in 22 of 25 biopsies (88%). In parallel sections stained by haematoxylin and eosin, parasites were detected in only 5 of 25 of the biopsies (20%). The demonstration of whole parasites or parasite antigen is diagnostic
Description
Keywords
leishmaniasis post kala-azar dermal leishmaniasis Leishmania donovani diagnosis immunoperoxidase staining monoclonal antibod