Molecular characterization of hepatitis B virus in liver disease patients and asymptomatic carriers of the virus in Sudan
| dc.Faculty | Endemic Diseases | en_US |
| dc.contributor.author | Yousif, Mukhlid | |
| dc.contributor.author | Mudawi, Hatim | |
| dc.contributor.author | Bakhiet, Sahar | |
| dc.contributor.editor | en_US | |
| dc.contributor.other | Molecular Biology | en_US |
| dc.date | 2013 | |
| dc.date.accessioned | 2015-11-23T06:52:53Z | |
| dc.date.available | 2015-11-23T06:52:53Z | |
| dc.date.issued | 2015-11-23 | |
| dc.date.submitted | 2015-11-23 | |
| dc.description.abstract | Background: Hepatitis B virus is hyperendemic in Sudan. Our aim was to molecularly characterize hepatitis B virus from Sudanese individuals, with and without liver disease, because genotypes play an important role in clinical manifestation and treatment management. Methods: Ninety-nine patients - 30 asymptomatic, 42 cirrhotic, 15 with hepatocellular carcinoma, 7 with acute hepatitis and 5 with chronic hepatitis- were enrolled. Sequencing of surface and basic core promoter/precore regions and complete genome were performed. Results: The mean ± standard deviation, age was 45.7±14.8 years and the male to female ratio 77:22. The median (interquartile range) of hepatitis B virus DNA and alanine aminotransferase levels were 2.8 (2.2-4.2) log IU/ml and 30 (19 – 49) IU/L, respectively. Using three genotyping methods, 81/99 (82%) could be genotyped. Forty eight percent of the 99 patients were infected with genotype D and 24% with genotype E, 2% with putative D/E recombinants and 7% with genotype A. Patients infected with genotype E had higher frequency of hepatitis B e antigen-positivity and higher viral loads compared to patients infected with genotype D. Basic core promoter/precore region mutations, including the G1896A in 37% of HBeAg-negative individuals, could account for hepatitis B e antigen-negativity. Pre-S deletion mutants were found in genotypes D and E. Three isolates had the vaccine escape mutant sM133T. Conclusion: Sudanese hepatitis B virus carriers were mainly infected with genotypes D or E, with patients infected with genotype E having higher HBeAg-positivity and higher viral loads. This is the first study to molecularly characterize hepatitis B virus from liver disease patients in Sudan | en_US |
| dc.identifier.uri | http://khartoumspace.uofk.edu/123456789/17262 | |
| dc.publisher | UOFK | en_US |
| dc.subject | Bioinformatics | en_US |
| dc.subject | Genotype | en_US |
| dc.subject | Serotype | en_US |
| dc.subject | Sudan | en_US |
| dc.subject | Subgenotype | en_US |
| dc.subject | Africa | en_US |
| dc.subject | Phylogenetics | en_US |
| dc.title | Molecular characterization of hepatitis B virus in liver disease patients and asymptomatic carriers of the virus in Sudan | en_US |
| dc.type | Article | en_US |